The CUR compound was purchased from Sigma-Aldrich Productions GmbH (Steinheim am Albuch, Germany). The CUR/γ-CD and CUR/HP-γ-CD complexes as pure forms (98% purity) were prepared according to the following protocols: 45 g of γ-CD and 5.0 g of CUR were weighed in a mortar and suspended in 25 ml distilled water with a pestle. Then 1.0 g of HP-γ-CD and 44 mg of CUR were weighed in a mortar and suspended in 1.5 ml of 96% ethanol with a pestle. After a complete homogenization, the suspension was dried in vacuo under ambient conditions for 3three days. Finally, the dry complexes were pulverized in the mortar. The CUR concentrations were determined by UV/Vis spectrophotometry using an Agilent 8453 instrument. The samples were adequately diluted with a 50 Vol% ethanol-water mixture to yield an absorbance in measurable range at 430 nm. All the CUR measurements were performed without any disturbing effects from CDs presented in the complex.

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